Illustration of the steps involved in Swine Liver hepatocytes cell isolation.

The schematic representation of sequential processes involved in the isolation of primary hepatocytes from Swine Liver. The liver tissue is collected and perfused to remove blood. The tissues are thoroughly washed, followed by mechanical disintegration and enzymatic digestion. The enzyme used is either collagenase or trypsin, which dissociate the extracellular matrix and release hepatocytes from the tissue structure. The cell suspension is further filtered and centrifuged to isolate a single cell suspension. The cells are seeded in a culture flask/petri dish in a suitable medium (consisting of all macronutrients and micronutrients). The cells are isolated at 37°C in the presence of 5% CO2 and 95% humidity. The cells are observed at specific time intervals. On reaching 80-85% confluency, the cells are subcultured and used for experimentation.

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